Product Detail
Product NameRb(Phospho-Ser780) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Rb only when phosphorylated at serine 780.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamesP105-RB; PP105; PP110; RB-1; RB1
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody #11132.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody #11132(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser780) Antibody #11132.
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.
Huang H, et al.(2004)Oncogene. 23(12): 2161-2176.
Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.
If you have published an article using product 11132, please notify us so that we can cite your literature.
et al,Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells.
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et al,Palbociclib inhibits epithelial-mesenchymal transition and metastasis in breast cancer via c-Jun/COX-2 signaling pathway.In Oncotarget on 2015 Dec 8 by Ge Qin, Fei Xu et al..PMID: 26540629
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et al,The potent novel CDK4/6 inhibitor TQB3616 in hormone receptor positive breast cancer: preclinical characterization with in vitro and human tumor xenograft modelsInBreast Cancer?On2023 Dec 8byWenyu Hu?#?1,?Lei Wang et al..PMID:?38090281
, (2023),
PMID:
38090281
Yes
