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CDK2(Phospho-Thr160) Antibody#11133

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Product Detail

Product NameCDK2(Phospho-Thr160) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from Human CDK2.

Target NameCDK2

ConjugateUnconjugated

Other Nameskinase Cdk2; p33 protein kinase;

Accession NoSwiss-Prot: P24941
NCBI Protein: NP_001789.2

Uniprot P24941

Gene ID 1017;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDK2(Phospho-Thr160) Antibody #11133.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK2(Phospho-Thr160) Antibody #11133(left) or the same antibody preincubated with blocking peptide(right).
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14.

If you have published an article using product 11133, please notify us so that we can cite your literature.

et al,Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.In Antioxid Redox Signal on 2017 Jul 10 by Xiao-Jing Shi , Lina Ding,et al..PMID: 27650197 , (2017),
PMID: 27650197
et al,Jaridonin-induced G2/M Phase Arrest in Human Esophageal Cancer Cells Is Caused by Reactive Oxygen Species-Dependent Cdc2-tyr15 Phosphorylation via ATM-Chk1/2-Cdc25C Pathway .In Toxicol Appl Pharmacol on 2015 Jan 15 by Yong-Cheng Ma , Nan Su et al..PMID:25450480 , (2015),
PMID: 25450480

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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