Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > CDC2(Phospho-Thr161) Antibody

CDC2(Phospho-Thr161) Antibody#11134

Availability: Yes
Total Amount:$
Product Detail

Product NameCDC2(Phospho-Thr161) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB;IHC;IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine161 (T-Y-T(p)-H-E) derived from Human CDC2.

Target NameCDC2

ConjugateUnconjugated

Other NamesCDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;

Accession NoSwiss-Prot: P06493
NCBI Protein: NP_001163877.1

Uniprot P06493

Gene ID 983;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300.  IF 1:50-200

Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Thr161) Antibody #11134.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Phospho-Thr161) Antibody #11134(left) or the same antibody preincubated with blocking peptide(right).

cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

If you have published an article using product 11134, please notify us so that we can cite your literature.

et al,Adaptive Gene Regulation of Pyruvate Dehydrogenase Kinase Isoenzyme 4 in Hepatotoxic Chemical-Induced Liver Injury and Its Stimulatory Potential for DNA Repair and Cell Proliferation.In J Recept Signal Transduct Res on 2011 Feb by Minori Dateki, Megumi Kunitomo,et al..PMID:21182459 , (2011),
PMID: 21182459
et al,Sinularin induces oxidative stress鈥恗ediated G2/M arrest and apoptosis in oral cancer cells.In Environ Toxicol on 2017 Sep by Yung-Ting Chang , Chang-Yi Wu,et al..PMID:28548367 , (2017),
PMID: 28548367
et al,Enhanced Proliferation of Bone Marrow Mesenchymal Stem Cells by Co-Culture With TM4 Mouse Sertoli Cells: Involvement of the EGF/PI3K/AKT Pathway .In Mol Cell Biochem on 2014 Aug byHuan Tian , Meijin Guo et al..PMID:24748323 , (2014),
PMID: 24748323

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;