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FKHR(Phospho-Ser319) Antibody#11136

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Product NameFKHR(Phospho-Ser319) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of FKHR only when phosphorylated at serine 319.

Immunogen TypePeptide-KLH

Immunogen DescThe antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser319.

Target NameFKHR

ConjugateUnconjugated

Other Names FOXO1

Accession NoSwiss-Prot: Q12778
NCBI Protein: NP_002006.2

Uniprot Q12778

Gene ID 2308;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:50-200

Western blot analysis of extracts from 3T3 cells untreated or treated with serum starvation using FKHR(Phospho-Ser319) Antibody #11136.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR(Phospho-Ser319) Antibody #11136(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using FKHR(Phospho-Ser319) Antibody #11136.

FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

Rena G, et al. (2002) EMBO J 21(9): 2263-2271.
Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607.
Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.

If you have published an article using product 11136, please notify us so that we can cite your literature.

et al,Activation of FoxO by LRRK2 induces expression of proapoptotic proteins and alters survival of postmitotic dopaminergic neuron in Drosophila. In Hum Mol Genet on 2010 Oct 1 by Tomoko Kanao, Katerina Venderova, et al..PMID:20624856 , (2010),
PMID: 20624856
et al,The nitric oxide-cyclic GMP pathway regulates FoxO and alters dopaminergic neuron survival in Drosophila. In PLoS One on 2012 by Tomoko Kanao, Tomoyo Sawada, et al..PMID:22393355 , (2012),
PMID: 22393355
et al,Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulate transcriptional repression of PCK1 in HepG2 cells.In Genes Cells on 2015 Apr by Tamaki Arai, Fumi Kano et al..PMID: 25736587 , (2015),
PMID: 25736587

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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