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cdc25A(Phospho-Ser76) Antibody#11138

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Product Detail

Product Namecdc25A(Phospho-Ser76) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A.

Target Namecdc25A

ConjugateUnconjugated

Other NamesCDC25M3; MPIP1;

Accession NoSwiss-Prot: P30304
NCBI Protein: NP_001780.2

Uniprot P30304

Gene ID 993;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A(Phospho-Ser76) Antibody #11138.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A(Phospho-Ser76) Antibody #11138(left) or the same antibody preincubated with blocking peptide(right).
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

If you have published an article using product 11138, please notify us so that we can cite your literature.

et al,CK1娄脜 targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation , (2011),
PMID: 21252624
et al,CK1蔚 targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation. In Cell Cycle on 2011 Feb 1 by Shunfu Piao, Su-Jin Lee, et al..PMID: 21252624 , (2011),
PMID: 21252624

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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