Product Detail
Product Namecofilin(Phospho-Ser3) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Target Namecofilin
ConjugateUnconjugated
Other NamesCFL; CFL1;
Accession NoSwiss-Prot: P23528
NCBI Protein: NP_005498.1
Uniprot
P23528
Gene ID
1072;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody #11139(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody #11139(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody #11139.
Lane 1 : HUVEC;
Lane 2 : HUVEC treated with EGF;
Lane 3 : MCF;
Lane 4 : MCF treated with EGF;
Lysates/proteins at 40 µg per lane.
Predicted band size : 19 kDa Observed band size : 19 kDa
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.
Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
If you have published an article using product 11139, please notify us so that we can cite your literature.
et al,Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.
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PMID:
23203801
et al,Downregulation of p57 accelerates the growth and invasion of hepatocellular carcinoma.
, (2011),
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22002319
et al,Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis.
, (2011),
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21205790
et al,Downregulation of p57 Accelerates the Growth and Invasion of Hepatocellular Carcinoma.In Carcinogenesis on 2011 Dec by Hui Guo ,
Yi Lv,et al..PMID: 22002319
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22002319
et al,Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis. In Development on 2011 Feb by Lijun Zhang, Jun Luo, et al..PMID:
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et al,Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool. In J Cell Sci on 2013 Jan 15 by Tiina Viita, Kari-Pekka Skarp,et al..PMID: 23203801
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23203801
et al,The role of cytoplasmic p57 in invasion of hepatocellular carcinoma.In BMC Gastroenterol. On 2015 Aug 15 by Hui Guo, Yi Li et al..PMID:26271467
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et al,Genome-wide RNAi Screen for Nuclear Actin Reveals a Network of Cofilin Regulators .In J Cell Sci on 2015 Jul 1 by Joseph Dopie, Eeva K Rajakyl盲 et al..PMID: 26021350
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PMID: 26021350
et al,Suppression of p21Rac Signaling and Increased Innate Immunity Mediate Remission in Crohn鈥檚 Disease.In sci Transl Med on 2014 Apr 23 by Kaushal Parikh , Lu Zhou et al..PMID:24760188
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PMID: 24760188
et al,The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in聽Drosophila聽mushroom body neurons via the non-canonical Rac-Cofilin pathway.In Development on 2014 Dec by Takashi Abe, Daisuke Yamazaki et al..PMID:25411210
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PMID: 25411210
et al,X chromosome-linked intellectual disability protein PQBP1 associates with and regulates the translation of specific mRNAs. In
Hum Mol Genet on 2015 Aug 15 by Didi Wan, Zi Chao Zhang,et al..PMID:26002102
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PMID: 26002102
et al,Scribble scaffolds a signalosome for active forgetting. In Neuron on 2016 Jun 15 by Isaac Cervantes-Sandoval, Molee Chakraborty,et al..PMID:27263975
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PMID: 27263975
et al,LIM Kinase Regulation of Cytoskeletal Dynamics Is Required for Salivary Gland Branching Morphogenesis. In Mol Biol Cellon 2014 Aug 15 by Shayoni Ray, Joseph A Fanti锛宔t al..PMID:24966172
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PMID: 24966172
et al,Regulation of nuclear actin levels and MRTF/SRF target gene expression during PC6.3 cell differentiation. In Exp Cell Res on 2022 Nov 15 by Salla Kyher?inen, Alise Hyrskyluoto, et al..PMID: 36122768
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