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Vav(Phospho-Tyr174) Antibody#11142

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Product Detail

Product NameVav(Phospho-Tyr174) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Rt

SpecificityThe antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 174 (E-I-Y(p)-E-D) derived from Human Vav.

Target NameVav

ConjugateUnconjugated

Other NamesVAV

Accession NoSwiss-Prot: P15498
NCBI Protein: NP_005419.2

Uniprot P15498

Gene ID 7409;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 95kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using Vav(Phospho-Tyr174) Antibody #11142.
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation

Amarasinghe GK, et al.

If you have published an article using product 11142, please notify us so that we can cite your literature.

et al,Signal-Transducing Adaptor Protein-2 Regulates Stromal Cell-Derived Factor-1 -Induced Chemotaxis in T Cells. , (2009),
PMID: 19933863
et al,Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1伪-induced chemotaxis in T cells. In J Immunol on 2009 Dec 15 by Yuichi Sekine, Osamu Ikeda, et al..PMID: 19933863 , (2009),
PMID: 19933863

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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