Product NameMKK3(Phospho-Ser189) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of MKK3 only when phosphorylated at serine189.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3.

Target NameMKK3

ConjugateUnconjugated

Other NamesMAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3

Accession NoSwiss-Prot: P46734
NCBI Protein: NP_002747.2

Uniprot P46734

Gene ID 5606;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200