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MKK6(Phospho-Ser207) Antibody#11146

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Product Detail

Product NameMKK6(Phospho-Ser207) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of MKK6 only when phosphorylated at serine207.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 207 (V-D-S(p)-V-A) derived from Human MKK6.

Target NameMKK6

ConjugateUnconjugated

Other NamesMAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK 6; MEK6

Accession NoSwiss-Prot: P52564
NCBI Protein: NP_002749.2

Uniprot P52564

Gene ID 5608;

Calculated MW37kD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:50-200

Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) Antibody #11146.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6(Phospho-Ser207) Antibody #11146(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using MKK6(Phospho-Ser207) Antibody #11146.

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008],

If you have published an article using product 11146, please notify us so that we can cite your literature.

et al,Suppression of phospho-p85α?CGTP-Rac1 lipid raft interaction by bichalcone analog attenuates cancer cell invasion. In Mol Carcinog on 2016 Dec by Hui-Li Lu, Shih-Shun Chen, et al..PMID: 26756739 , (2016),
PMID: 26756739

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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