Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > JAK1(Phospho-Tyr1034) Antibody

JAK1(Phospho-Tyr1034) Antibody#11149

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(9) Other
Product Detail

Product NameJAK1(Phospho-Tyr1034) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF;WB;IP;IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1034.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1034 (K-E-Y(p)-Y-T) derived from Human JAK1.

Target NameJAK1

ConjugateUnconjugated

Other Names Janus kinase 1

Accession NoSwiss-Prot: P23458
NCBI Protein: NP_002218.2

Uniprot P23458

Gene ID 3716;

Calculated MW132kD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:200-1:1000;
IHC 1:100-1:300;
IF 1:50-200

Western Blot analysis of various cells using Phospho-JAK1 (Y1034) Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from A549 cells , using JAK1 (Phospho-Tyr1034) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

If you have published an article using product 11149, please notify us so that we can cite your literature.

et al,Zap70 Functions to Maintain Stemness of Mouse Embryonic Stem Cells by Negatively Regulating Jak1/Stat3/c-Myc Signaling. , (2010),
PMID: 20641039
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861 , (2019),
PMID: 31594861
et al,Zap70 functions to maintain stemness of mouse embryonic stem cells by negatively regulating Jak1/Stat3/c鈥怣yc signaling.In Stem Cells on 2010 Sep by Young Cha , Bo-hyun Moon, et al..PMID:20641039 , (2010),
PMID: 20641039
et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. In J Cancer Res Clin Oncol on 2011 Nov by Dan Liu, Yi Huang, et al..PMID:21861134 , (2011),
PMID: 21861134
et al,Notch-induced hIL-6 production facilitates the maintenance of self-renewal of hCD34+ cord blood cells through the activation of Jak-PI3K-STAT3 pathway. In Am J Pathol on 2012 Jan by Bongkum Choi, Eunyoung Chun, et al..PMID:22062221 , (2012),
PMID: 22062221
et al,Activation of Janus kinase 1 confers poor prognosis in patients with non鈥憇mall cell lung cancer.In Oncol Lett on 2017 Oct by Dan Liu, Yi Huang ,et al..PMID: 28989534 , (2017),
PMID: 28989534
et al,Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. In Toxicol Appl Pharmacol on 2020 Oct 1 by Yuwen Sheng, Zhonghui Zhang, et al..PMID:32822738 , (2020),
PMID: 32822738
More
et al,A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase. In Int J Mol Sci on 2021 Mar 25 by Ting Peng, Orawan Wonganan, et al..PMID:33805945 , (2021),
PMID: 33805945
et al,Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase , (2017),
PMID: 29371942

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors