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Jak2(Phospho-Tyr1007) Antibody#11151

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Product Detail

Product NameJak2(Phospho-Tyr1007) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of JAK2 only when phosphorylated at tyrosine 1007.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1007 (K-E-Y(p)-Y-K) derived from Human JAK2.

Target NameJak2

ConjugateUnconjugated

Other NamesJAK-2; JAK2; Janus kinase 2; kinase Jak2;

Accession NoSwiss-Prot: O60674
NCBI Protein: NP_004963.1

Uniprot O60674

Gene ID 3717;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29 cells untreated or treated with IL-4 using JAK2(Phospho-Tyr1007) Antibody #11151.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK2(Phospho-Tyr1007) Antibody #11151(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of various lysates using Jak2(Phospho-Tyr1007) Antibody Antibody at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution. Lysates/proteins: 25μg per lane.

Plays a role in leptin signaling and control of body weight

James C, et al. (2005) Nature. 434(7037): 1144-1148.
Argetsinger LS, et al. (2004) Mol Cell Biol. 24(11): 4955-4967.
Ungureanu D, et al. (2002) Mol Cell Biol. 22(10): 3316-3326.
Xie S, et al. (2001) Oncogene. 20(43): 6188-6195.

If you have published an article using product 11151, please notify us so that we can cite your literature.

et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861 , (2019),
PMID: 31594861
et al,The CombInation of Adipose-derived Schwann-like Cells and Acellular Nerve Allografts Promotes Sciatic Nerve Regeneration and Repair through the JAK2/STAT3 SignalIng Pathway In Rats. In Neuroscience on 2019 Dec 1 by Fu XM, Wang Y, et al..PMID:31682951 , (2019),
PMID: 31682951
et al,Cetuximab enhances oridonin-induced apoptosis through mitochondrial pathway and endoplasmic reticulum stress in laryngeal squamous cell carcinoma cells. In Toxicol In Vitro on 2020 May 12 by Ning Kang, Shijie Cao, et al..PMID:32407876 , (2020),
PMID: 32407876

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;