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Location： Home > Phospho Antibodies > Src(Phospho-Tyr529) Antibody

Src(Phospho-Tyr529) Antibody#11153

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Product Detail

Product NameSrc(Phospho-Tyr529) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.

Target NameSrc

ConjugateUnconjugated

Other NamesC-SRC; SRC1;

Accession NoSwiss-Prot: P12931
NCBI Protein: NP_005408.1

Uniprot P12931

Gene ID 6714;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody #11153.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody #11153(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody #11153.
Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody #11153.

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838
Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040
Xu W., (1997).Nature 385:595-602
Benes C.H., (2005) Cell 121:271-280

If you have published an article using product 11153, please notify us so that we can cite your literature.

et al,FAK Phosphorylation by ERK Primes Ras-Induced Tyrosine Dephosphorylation of FAK Mediated by PIN1 and PTP-PEST. , (2009),
PMID: 19595712
et al,FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST. In Mol Cell on 2009 Jul 10 by Yanhua Zheng, Yan Xia, et al..PMID:19595712 , (2009),
PMID: 19595712
et al,Identification of Demethylincisterol A 3 as a Selective Inhibitor of Protein Tyrosine Phosphatase Shp2.In Eur J Pharmacol on 2017 Jan 15 by Chuan Chen , Fan Liang,et al..PMID: 27939989 , (2017),
PMID: 27939989
et al,Discovery of a novel inhibitor of the protein tyrosine phosphatase Shp2.In Sci Rep on 2015 Dec 2 by Chuan Chen , Mengmeng Cao et al..PMID: 26626996 , (2015),
PMID: 26626996
et al,Neuronal deletion of GSK3尾 increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2.In MC Biol on 2014 Jun 12 by M谩rcia A Liz, Fernando M Mar et al..PMID: 24923837 , (2014),
PMID: 24923837
et al,Discovery of a Novel Src Homology-2 Domain Containing Protein Tyrosine Phosphatase-2 (SHP2) and Cyclin-Dependent Kinase 4 (CDK4) Dual Inhibitor for the Treatment of Triple-Negative Breast Cancer , (2022),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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