Product NamePAK1(Phospho-Thr212) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 212 (P-V-T(p)-P-T) derived from Human PAK1.

Target NamePAK1

ConjugateUnconjugated

Other Namesp21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;

Accession NoSwiss-Prot: Q13153
NCBI Protein: NP_001122092.1

Uniprot Q13153

Gene ID 5058;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200