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Location： Home > Phospho Antibodies > eNOS(Phospho-Ser1177) Antibody

eNOS(Phospho-Ser1177) Antibody#11156

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Product Detail

Product NameeNOS(Phospho-Ser1177) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB;IF

Species ReactivityHu Ms Rt

SpecificityPhospho-NOS3 (S1177) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S1177.

Immunogen TypePeptide-KLH

Immunogen DescThe antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176

Target NameeNOS

ConjugateUnconjugated

Other NamesConstitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII

Accession NoSwiss-Prot: P29474
NCBI Protein: NP_000594.2

Uniprot P29474

Gene ID 4846;

Calculated MW130-140kD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:2000. IF 1:200 - 1:1000.

Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using eNOS (Phospho-Ser1176) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

If you have published an article using product 11156, please notify us so that we can cite your literature.

et al,Chronic intermittent hypobaric hypoxia protects vascular endothelium by ameliorating autophagy in metabolic syndrome rats. In Life Sci.On 2018 Jul 15 by Cui F, Guan Y et al..PMID:29733850 , (2018),
PMID: 29733850
et al,Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation.In J Transl Med. On 2018 Dec 18 by Harding IC, Mitra R et al..PMID: 30563532 , (2018),
PMID: 30563532
et al,Gardenamide A protects RGC-5 cells from H2O2-induced oxidative stress insults by activating PI3K/Akt/eNOS signaling pathway.In Int J Mol Sci on 2015 Sep 15 by Rikang Wang , Lizhi Peng et al..PMID:26389892 , (2015),
PMID: 26389892
et al,Chaiqi decoction ameliorates vascular endothelial injury in metabolic syndrome by upregulating autophagy. In Am J Transl Res on 2020 Sep 15 by Xun Chen, Xiao-Ru Yan,et al..PMID:33042397 , (2020),
PMID: 33042397
et al,Adenosine mono-phosphate-activated protein kinase-mammalian target of rapamycin signaling participates in the protective effect of chronic intermittent hypobaric hypoxia on vascular endothelium of metabolic syndrome rats. In Chin J Physiol on 2022 Mar-Apr by Fang Cui, Min Shi,et al..PMID:35488670 , (2022),
PMID: 35488670

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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