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FKHRL1(Phospho-Ser253) Antibody#11157

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Product Detail

Product NameFKHRL1(Phospho-Ser253) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.

Target NameFKHRL1

ConjugateUnconjugated

Other NamesFOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Accession NoSwiss-Prot: O43524
NCBI Gene ID: 2309
NCBI mRNA: NM_001455.3
NCBI Protein: NP_001446.1

Uniprot O43524

Gene ID 2309;

Calculated MW90kD

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C

Application Details

Predicted MW: 97kd

Western blotting: 1:500~1:1000

Immunohistochemistry: 1:50~1:100

Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from NIH/3T3 cells using FKHRL1 (Ab-253) antibody (#21171, Lane 1 and 2) and FKHRL1 (phospho-Ser253) antibody (#11157, Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody (#11157).
Immunofluorescence staining of methanol-fixed HeLa cells using FKHRL1 (phospho-Ser253) antibody (#11157, Red).
Western Blot analysis of HELA cells using Phospho-FoxO3A (S253) Polyclonal Antibody diluted at 1:2000

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

 

If you have published an article using product 11157, please notify us so that we can cite your literature.

et al,Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats.In J Neurochem on 2010 Aug by Zhan L, Wang T, et al..PMID:20492357 , (2010),
PMID: 20492357
et al,Activation of Akt/FoxO and inactivation of MEK/ERK pathways contribute to induction of neuroprotection against transient global cerebral ischemia by delayed hypoxic postconditioning in adult rats.In Neuropharmacology on 2012 Oct by Zhan L, Li D,et al..PMID:22749925 , (2012),
PMID: 22749925
et al,Involvement of PI3K/Akt/FoxO3a and PKA/CREB signaling pathways in the protective effect of fluoxetine against corticosterone-induced cytotoxicity in PC12 cells.In J Mol Neurosci on 2016 Aug by Bingqing Zeng , Yiwen Li et al ..PMID:27412469 , (2016),
PMID: 27412469

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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