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VASP(Phospho-Ser239) Antibody#11158

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Product Detail

Product NameVASP(Phospho-Ser239) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of VASP only when phosphorylated at serine 239.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 238 (K-V-S(p)-K-Q) derived from Human VASP.

Target NameVASP

ConjugateUnconjugated

Accession NoSwiss-Prot: P50552
NCBI Protein: NP_003361.1

Uniprot P50552

Gene ID 7408;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP(Phospho-Ser239) Antibody #11158.
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser239) Antibody #11158(left) or the same antibody preincubated with blocking peptide(right).
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Wang HG, et al.

If you have published an article using product 11158, please notify us so that we can cite your literature.

et al,Influence of short-term refrigeration on collagen-dependent signalling mechanisms in stored platelets. In Cell Signal on 2024 Oct by Julia Zeller-Hahn, Marius Bittl,et al..PMID:39048036 , (2024),
PMID: 39048036

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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