Product NameZap-70(Phospho-Tyr493) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 493 (S-Y-Y(p)-T-A) derived from Human Zap-70.

Target NameZap-70

ConjugateUnconjugated

Other Names70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70

Accession NoSwiss-Prot: P43403
NCBI Protein: NP _001070.2

Uniprot P43403

Gene ID 7535;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100