Product NameMEK1(Phospho-Ser221) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1.

Target NameMEK1

ConjugateUnconjugated

Other NamesERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1

Accession NoSwiss-Prot: Q02750
NCBI Protein: NP_002746.1

Uniprot Q02750

Gene ID 5604;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 45kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200