Product Detail
Product NameSTAT1(Phospho-Ser727) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E) derived from Human STAT1.
Target NameSTAT1
ConjugateUnconjugated
Other NamesISGF-3; STAT91;
Accession NoSwiss-Prot: P42224
NCBI Protein: NP_009330.1
Uniprot
P42224
Gene ID
6772;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using STAT1(Phospho-Ser727) Antibody #11163.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Phospho-Ser727) Antibody #11163(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using STAT1(Phospho-Ser727) Antibody #11163.
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Yuan ZM, et al. (1999) Nature.399 (6738): 814-817.
Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839.
Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903.
Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780.
If you have published an article using product 11163, please notify us so that we can cite your literature.
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