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PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody#11165

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Product Detail

Product NamePAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe Antibody detects endogenous level of PAK1/PAK2/PAK3 only when phosphorylated at threonine 423/402/421.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3.

Target NamePAK1/PAK2/PAK3

ConjugateUnconjugated

Other Namesp21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;

Accession NoSwiss-Prot:Q13153
Gene ID:5058

Uniprot Q13153

Gene ID 5058;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 61-67 (PAK2) 68-74 (PAK1/3)kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody #11165(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody #11165.

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

If you have published an article using product 11165, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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