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NMDAR2B(phospho-Tyr1474) Antibody#11168

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Product Detail

Product NameNMDAR2B(phospho-Tyr1474) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of NMDAR2B only when phosphorylated at Tyr1474.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Tyr1474 (H-V-Y(p)-E-K) derived from Human NMDAR2B.

Target NameNMDAR2B

ConjugateUnconjugated

Other NamesGRIN2B; NMDE2; NME2; NR2B; NR3

Accession NoSwiss-Prot: Q13224
NCBI Protein: NP_000825.2

Uniprot Q13224

Gene ID 2904;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 190kd

Western Blot: 1:500~1000
Immunofluorescence: 1:100~1:200

Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(phospho-Tyr1474) antibody #11168.
Western blot analysis of extracts from A431 cells untreated or treated with EGF using NMDAR2B (phospho-Tyr1474) Antibody #11168.

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Babb TL et al.Epilepsy Res. 2005 Mar-Apr;64(1-2):23-30
Chazot PL. Curr Med Chem. 2004 Feb;11(3):389-96
Kakegawa W, et al.Eur J Neurosci. 2003 Feb;17(4):887-91
 

If you have published an article using product 11168, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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