Product Detail
Product NamePKCth(Phospho-Ser695) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of PKCth only when phosphorylated at serine 695.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKCth.
Target NamePKCth
ConjugateUnconjugated
Other NamesKPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
Accession NoSwiss-Prot: Q04759
NCBI Protein: NP_006248.1
Uniprot
Q04759
Gene ID
5588;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 80kd
Western blotting: 1:500
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated or treated with PMA using PKCth(Phospho-Ser695) Antibody #11173.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser695) Antibody #11173(left) or the same antibody preincubated with blocking peptide(right).
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Xu ZB, et al.(2004) J Biol Chem 279:50401-50409
Thebault S, et al. (2004) Mol Immunol 40: 931-942
If you have published an article using product 11173, please notify us so that we can cite your literature.
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