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PLCg2(Phospho-Tyr753) Antibody#11175

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Product Detail

Product NamePLCg2(Phospho-Tyr753) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PLCg2 only when phosphorylated at tyrosine 753.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLCg2.

Target NamePLCg2

ConjugateUnconjugated

Other NamesPLC-IV; PLC-gamma2; Phospholipase C-gamma-2

Accession NoSwiss-Prot: P16885
NCBI Protein: NP_002652.2

Uniprot P16885

Gene ID 5336;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 150kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody #11175.
Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Phospho-Tyr753) Antibody #11175.
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999
Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661
Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029
Rodriguez R, et al. (2003) Biochem J 374 : 269-280

If you have published an article using product 11175, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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