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SEK1/MKK4(Phospho-Thr261) Antibody#11176

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Product Detail

Product NameSEK1/MKK4(Phospho-Thr261) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at threonine 261.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine261 (A-K-T(p)-RD) derived from Human SEK1/MKK4.

Target NameSEK1/MKK4

ConjugateUnconjugated

Other NamesJNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; MAPKK 4

Accession NoSwiss-Prot: P45985
NCBI Protein: NP_003001.1

Uniprot P45985

Gene ID 6416;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 44kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4(Phospho-Thr261) Antibody #11176(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Phospho-Thr261) Antibody #11176.
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403.
Leung IW, et al. (2001) J Biol Chem; 276(3): 1961-7.

If you have published an article using product 11176, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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