Product Name14-3-3z(Phospho-Ser58) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of 14-3-3 z only when phosphorylated at serine 58.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.

Target Name14-3-3z

ConjugateUnconjugated

Other Names1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1

Accession NoSwiss-Prot: P63104
NCBI Protein: NP_001129171.1

Uniprot P63104

Gene ID 7534;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 28kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200