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Histone H3.1(Phospho-Ser10) Antibody#11184

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Product NameHistone H3.1(Phospho-Ser10) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Histone H3.1 onlywhen phosphorylated at serine 10.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.

Target NameHistone H3.1

ConjugateUnconjugated

Other NamesH3/b, H3FB

Accession NoSwiss-Prot: P68431
NCBI Protein: NP_003521.2

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW17

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 17kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1(Phospho-Ser10) Antibody #11184(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.
Western blot analysis of extracts of various cell lines, using Histone H3.1(Phospho-Ser10) Antibody #11184

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Dai J, et al. (2005) Genes Dev 19(4): 472-488.
Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.
 

If you have published an article using product 11184, please notify us so that we can cite your literature.

et al,PKM2 Regulates Chromosome Segregation and Mitosis Progression of Tumor Cells , (2014),
PMID: 24316223
et al,The protective effect of Alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose , (2012),
PMID: 22796564
et al,PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. , (2012),
PMID: 22901803
et al,Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR娄脙/NF-娄脢B pathway , (2012),
PMID: 23199346
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803 , (2012),
PMID: 22901803
et al,Avian Blastoderm Dormancy Arrests Cells in G 2 and Suppresses Apoptosis.In FASEB J on 2017 Aug by Mee Hyun Ko Young Sun Hwang,et al..PMID: 28404741 , (2017),
PMID: 28404741
et al,UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer.In Sci Rep on 2014 Nov 7 by Shang-Ze Li , Yang Song et al..PMID: 25376843 , (2014),
PMID: 25376843

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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