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DARPP-32(Phospho-Thr75) Antibody#11185

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Product Detail

Product NameDARPP-32(Phospho-Thr75) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of DARPP-32 only when phosphorylated at threonine 75.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from Human DARPP-32.

Target NameDARPP-32

ConjugateUnconjugated

Other NamesIPPD; PPP1R1B;

Accession NoSwiss-Prot: Q9UD71
NCBI Protein: NP_115568.2

Uniprot Q9UD71

Gene ID 84152;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 32kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using DARPP-32(Phospho-Thr75) Antibody #11185.
Inhibitor of protein-phosphatase 1.

If you have published an article using product 11185, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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