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Ephrin-B2(Phospho-Tyr316) Antibody#11188

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Product Detail

Product NameEphrin-B2(Phospho-Tyr316) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2

Target NameEphrin-B2

ConjugateUnconjugated

Other NamesHTKL; EPLG5; Htk-L; LERK5;

Accession NoSwiss-Prot: P52799
NCBI Protein: NP_004084.1

Uniprot P52799

Gene ID 1948;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2(Phospho-Tyr316) Antibody #11188.
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588

If you have published an article using product 11188, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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