Product Detail
Product NameEphrin-B2(Phospho-Tyr330) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 330 (N-I-Y(p)-Y-K) derived from Human Ephrin-B2
Target NameEphrin-B2
ConjugateUnconjugated
Other NamesHTKL; EPLG5; Htk-L; LERK5; MGC126226
Accession NoSwiss-Prot: P52799
NCBI Protein: NP_004084.1
Uniprot
P52799
Gene ID
1948;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2(Phospho-Tyr330) Antibody #11189.
Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.
If you have published an article using product 11189, please notify us so that we can cite your literature.
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