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HDAC4(Phospho-Ser632) Antibody#11192

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Product Detail

Product NameHDAC4(Phospho-Ser632) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of HDAC4 only when phosphorylated at serine 632.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 632 (A-Q-S(p)-S-P) derived from Human HDAC4.

Target NameHDAC4

ConjugateUnconjugated

Other NamesHD4

Accession NoSwiss-Prot: P56524
NCBI Protein: NP_006028.2

Uniprot P56524

Gene ID 9759;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 140kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with EGF(lane 2) using HDAC4(Phospho-Ser632) Antibody #11192.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Wang AH, et al. (2000) Mol Cell Biol. 20(18): 6904-6912.
Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97(14): 7835-7840.
 

If you have published an article using product 11192, please notify us so that we can cite your literature.

et al,Distinct Mechanisms Involving Diverse Histone Deacetylases Repress Expression of the Two Gonadotropin ?-Subunit Genes in Immature Gonadotropes, and Their Actions Are Overcome by Gonadotropin-Releasing Hormone. , (2007),
PMID: 17371839

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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