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HSF1(Phospho-Ser307) Antibody#11195

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Product Detail

Product NameHSF1(Phospho-Ser307) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of HSF1 only when phosphorylated at serine 307.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1.

Target NameHSF1

ConjugateUnconjugated

Other NamesHSTF 1

Accession NoSwiss-Prot: Q00613
NCBI Protein: NP_005517.1

Uniprot Q00613

Gene ID 3297;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with TNF-a(lane 2) using HSF1(Phospho-Ser307) Antibody #11195.
Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody #11195.
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (Phospho-Ser307) Antibody #11195.

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

If you have published an article using product 11195, please notify us so that we can cite your literature.

et al,IER family proteIns are regulators of proteIn phosphatase PP2A and modulate the phosphorylation status of CDC25A. In Cell Signal on 2019 Mar by Ueda T, Kohama Y, et al.. PMID: 30599213 , (2019),
PMID: 30599213

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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