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HSP90B(Phospho-Ser254) Antibody#11196

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Product Detail

Product NameHSP90B(Phospho-Ser254) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B.

Target NameHSP90B

ConjugateUnconjugated

Other NamesHS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2

Accession NoSwiss-Prot: P08238
NCBI Protein: NP_031381.2

Uniprot P08238

Gene ID 3326;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 92kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody #11196.
Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody #11196.

Molecular chaperone. Has ATPase activity.

Zhao R, Houry WA.(2007) Adv Exp Med Biol; 594: 27-36.
Olsen JV, et al. (2006) Cell; 127(3): 635-648
Ogiso H, et al. (2004) Biochemistry; 43(49): 15510-15519

If you have published an article using product 11196, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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