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SMC1(Phospho-Ser957) Antibody#11198

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Product Detail

Product NameSMC1(Phospho-Ser957) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 957 (G-S-S(p)-Q-G) derived from Human SMC1.

Target NameSMC1

ConjugateUnconjugated

Other NamesSM1A; SMC1A; SMC1L1; SMC1 alpha protein;

Accession NoSwiss-Prot: Q14683
NCBI Protein: NP_006297.2

Uniprot Q14683

Gene ID 8243;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated or treated with UV using SMC1(Phospho-Ser957) Antibody #11198.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1(Phospho-Ser957) Antibody #11198(left) or the same antibody preincubated with blocking peptide(right).
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303.
Kim ST. et al. (2002) Genes Dev. 16(5): 560-570.
Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998

If you have published an article using product 11198, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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