Product Detail
                                
                                                                        Product NameBcr(Phospho-Tyr177) Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
                                        ApplicationsWB IHC
                                        Species ReactivityHu Ms
                                        SpecificityThe antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.
                                        Immunogen TypePeptide-KLH
                                         Immunogen DescPeptide sequence around phosphorylation site of tyrosine 177 (P-F-Y(p)-V-N) derived from Human Bcr.
                                         Target NameBcr
                                         ConjugateUnconjugated
                                                                                 Other NamesBCR; BCR protein; BCR1
                                        Accession NoSwiss-Prot: P11274
NCBI Protein: NP_004318.3 
                                        Uniprot
                                        P11274
                                        
                                        Gene ID
                                        613;
                                        
                                                                                                                        Concentration1.0mg/ml
                                         FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
                                        StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use. 
                                                                                                                                                         
                                Application Details
                                Predicted MW: 210kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
                             
                            
                                                                
                                    
                                    Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using Bcr(Phospho-Tyr177) Antibody #11199.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr(Phospho-Tyr177) Antibody #11199(left) or the same antibody preincubated with blocking peptide(right).
                                 
                                                             
                            
                                GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 
                            
                            
                                If you have published an article using product 11199, please notify us so that we can cite your literature.