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Bcr(Phospho-Tyr177) Antibody#11199

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Product Detail

Product NameBcr(Phospho-Tyr177) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 177 (P-F-Y(p)-V-N) derived from Human Bcr.

Target NameBcr

ConjugateUnconjugated

Other NamesBCR; BCR protein; BCR1

Accession NoSwiss-Prot: P11274
NCBI Protein: NP_004318.3

Uniprot P11274

Gene ID 613;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 210kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using Bcr(Phospho-Tyr177) Antibody #11199.
Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr(Phospho-Tyr177) Antibody #11199(left) or the same antibody preincubated with blocking peptide(right).
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907

If you have published an article using product 11199, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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