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Paxillin(Phospho-Tyr31) Antibody#11201

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Product Detail

Product NamePaxillin(Phospho-Tyr31) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 31 (T-P-Y(p)-S-Y) derived from Human Paxillin.

Target NamePaxillin

ConjugateUnconjugated

Other NamesPAXI; PXN;

Accession NoSwiss-Prot: P49023
NCBI Protein: NP_001074324.1

Uniprot P49023

Gene ID 5829;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 68kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Paxillin(Phospho-Tyr31) Antibody #11201.

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J 20(13): 3414-3426.
Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96(3): 1123-1128.
Goldberg MB. (2001) Microbiol Mol Biol Rev 65(4): 595-626.
Kook S, et al. (2000) Mol Biol Cell 11(3): 929-939.

If you have published an article using product 11201, please notify us so that we can cite your literature.

et al,JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin. , (2008),
PMID: 18713649
et al,JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin.In Cell Signal on 2008 Nov by Zhi Huang , Da-Peng Yan, et al..PMID:18713649 , (2008),
PMID: 18713649
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et al..PMID: 23665237 , (2013),
PMID: 23665237
et al,Discovery of a novel inhibitor of the protein tyrosine phosphatase Shp2.In Sci Rep on 2015 Dec 2 by Chuan Chen , Mengmeng Cao et al..PMID: 26626996 , (2015),
PMID: 26626996

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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