Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > Rac1(Phospho-Ser71) Antibody

Rac1(Phospho-Ser71) Antibody#11203

Availability: Yes
Total Amount:$
Product Detail

Product NameRac1(Phospho-Ser71) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Rac1 only when phosphorylated at serine 71.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 71 (P-L-S(p)-Y-P) derived from Human Rac1.

Target NameRac1

ConjugateUnconjugated

Other NamesRas-like protein TC25; Ras-related C3 botulinum toxin substrate 1; p21-Rac1

Accession NoSwiss-Prot: P63000
NCBI Protein: NP_008839.2

Uniprot P63000

Gene ID 5879;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 28kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with PMA(lane 2) using Rac1(Phospho-Ser71) Antibody #11203.
Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

If you have published an article using product 11203, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;