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p21Cip1(Phospho-Thr145) Antibody#11206

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Product Detail

Product Namep21Cip1(Phospho-Thr145) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of p21Cip1 only when phosphorylated at threonine 145.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 145 (R-Q-T(p)-S-M) derived from Human p21Cip1.

Target Namep21Cip1

ConjugateUnconjugated

Other NamesCAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A

Accession NoSwiss-Prot: P38936
NCBI Protein: NP _000380.1

Uniprot P38936

Gene ID 1026;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 21kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p21Cip1(Phospho-Thr145) Antibody #11206(left) or the same antibody preincubated with blocking peptide(right).
May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

Ocker M Int,et al. (2007)J Biochem Cell Biol. 39(7-8):1367-74.
Dangi S, et al.(2006)Cell Prolif. 39(4):261-79.
Chen J,et al.(2006)Am J Physiol Heart Circ Physiol. 290(4):H1575-86

If you have published an article using product 11206, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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