Product Namep27Kip1(Phospho-Thr187) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.

Target Namep27Kip1

ConjugateUnconjugated

Other NamesCDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1

Accession NoSwiss-Prot: P46527
NCBI Protein: NP_004055.1

Uniprot P46527

Gene ID 1027;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100