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Synaptotagmin 1/2 (Phospho-Thr202/199) Antibody#11210

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Product Detail

Product NameSynaptotagmin 1/2 (Phospho-Thr202/199) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Synaptotagmin 1/2 only when phosphorylated at threonine202/199.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 202/199 (R-K-T(p)-L-N) derived from Human Synaptotagmin 1/2.

Target NameSynaptotagmin 1/2

ConjugateUnconjugated

Other NamesSYT1/2; SytI/II; Synaptotagmin I/II; P65; SYT

Accession NoSwiss-Prot:P21579
Gene ID:6857

Uniprot P21579

Gene ID 6857;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 46kd
Immunofluorescence: 1:100~1:200

Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1/2 (Phospho-Thr202/199) Antibody #11210.

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse

Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.

If you have published an article using product 11210, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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