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Tyrosine Hydroxylase(Phospho-Ser40) Antibody#11212

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Product Detail

Product NameTyrosine Hydroxylase(Phospho-Ser40) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Tyrosine Hydroxylase only when phosphorylated at serine 40.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 40 (R-Q-S(p)-L-I) derived from Human Tyrosine Hydroxylase (TH).

Target NameTyrosine Hydroxylase

ConjugateUnconjugated

Other NamesTY3H; TYH; Tyrosine 3-hydroxylase; tyrosine hydroxylase;

Accession NoSwiss-Prot: P07101
NCBI Protein: NP_000351.2

Uniprot P07101

Gene ID 7054;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 55-60 kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using Tyrosine Hydroxylase(Phospho-Ser40) Antibody #11212.
The protein encoded by Tyrosine Hydroxylase is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Vazin T, et al. Stem Cells. 2008 Jun;26(6):1517-25
Pistocchi A, et al. BMC Dev Biol. 2008 Mar 10;8:27
Fukakusa A, et al. J Pharmacol Sci. 2008 Feb;106(2):321-4.

If you have published an article using product 11212, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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