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IkB-e(Phospho-Ser22) Antibody#11213

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Product Detail

Product NameIkB-e(Phospho-Ser22) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of IkB-e only when phosphorylated at serine 22.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Mouse IkB-ε.

Target NameIkB-e

ConjugateUnconjugated

Other NamesI-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon

Accession NoSwiss-Prot: O54910
NCBI Gene ID: 18037
NCBI Protein: NP_004547.2

Uniprot O54910

Gene ID 18037;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody #11213.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Phospho-Ser22) Antibody #11213(left) or the same antibody preincubated with blocking peptide(right).

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.

If you have published an article using product 11213, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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