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b-Catenin(Phospho-Ser33) Antibody#11218

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Product Detail

Product Nameb-Catenin(Phospho-Ser33) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of b-Catenin only when phosphorylated at serine 33.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human b-Catenin.

Target Nameb-Catenin

ConjugateUnconjugated

Other NamesCTNNB1; CATNB; CTNB1; CTNNB;

Accession NoSwiss-Prot: P35222
NCBI Protein: NP_001091679.1

Uniprot P35222

Gene ID 1499;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) Antibody #11218.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody #11218(left) or the same antibody preincubated with blocking peptide(right).
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952

If you have published an article using product 11218, please notify us so that we can cite your literature.

et al,Effect of Small-molecule GSK3 Antagonist on Differentiation of Rat Dental Pulp Cells into Odontoblasts.In In Vivo on 2020 May-Jun by Masuda Y, Sakagami H, et al..PMID: 32354894 , (2020),
PMID: 32354894

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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