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Location： Home > Phospho Antibodies > EGFR(Phospho-Tyr1172) Antibody

EGFR(Phospho-Tyr1172) Antibody#11220

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Product Detail

Product NameEGFR(Phospho-Tyr1172) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level EGFR only when phosphorylated at tyrosine 1172.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q) derived from Human EGFR.

Target NameEGFR

ConjugateUnconjugated

Other NamesERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR

Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2

Uniprot P00533

Gene ID 1956;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR(Phospho-Tyr1172) Antibody #11220.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Tyr1172) Antibody #11220(left) or the same antibody preincubated with blocking peptide(right).
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Noguchi T, et al. (1994) Mol Cell Biol 14(10): 6674-6682
Doherty JK, et al. (1999) Proc Natl Acad Sci U S A 96(19): 10869-10874
Kanner SB, et al. (1991) Mol Cell Biol 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell 9(7): 1661-1674
O

If you have published an article using product 11220, please notify us so that we can cite your literature.

et al,ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. , (2012),
PMID: 23178880
et al,Epidermal Growth Factor (EGF)-enhanced Vascular Cell Adhesion molecule-1 (VCAM-1) Expression Promotes Macrophage and Glioblastoma Cell Interaction and Tumor Cell Invasion.In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang,et al..PMID:24045955 , (2013),
PMID: 24045955
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by Weiwei Yang, Yan Xia, et al..PMID: 23123196 , (2012),
PMID: 23123196
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803 , (2012),
PMID: 22901803
et al,EGF-enhanced vascular cell adhesion molecule-1 (VCAM-1) expression promotes macrophage and glioblastoma cell interaction and tumor cell invasion. In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang, et al..PMID: 24045955 , (2013),
PMID: 24045955
et al,Tumor-penetrating Peptide Fused EGFR Single-Domain Antibody Enhances Cancer Drug Penetration Into 3D Multicellular Spheroids and Facilitates Effective Gastric Cancer Therapy .In J Control Release on 2015 Feb 28 by Huizi Sha , Zhengyun Zou et al..PMID: 25553823 , (2015),
PMID: 25553823
et al,EGFR phosphorylation of DCBLD2 recruits TRAF6 and stimulates AKT-promoted tumorigenesis. In J Clin Invest on 2014 Sep by An-Jey A Su, Philip E Auron, et al..PMID:25061874 , (2014),
PMID: 25061874
More
et al,Epidermal growth factor (EGF)-enhanced vascular cell adhesion molecule-1 (VCAM-1) expression promotes macrophage and glioblastoma cell interaction and tumor cell invasion.. In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang, et al..PMID:24045955 , (2013),
PMID: 24045955

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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