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4E-BP1(Phospho-Thr37) Antibody#11222

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Product Detail

Product Name4E-BP1(Phospho-Thr37) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 36.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 37(S-T-T(p)-P-G) derived from Human 4E-BP1.

Target Name4E-BP1

ConjugateUnconjugated

Other NamesBP-1; 4EBP1; 4E-BP1; PHAS-I;

Accession NoSwiss-Prot: Q13541; NCBI Gene ID: 1978; NCBI mRNA: NM_004095.3; NCBI Protein: NP_004086.1

Uniprot Q13541

Gene ID 1978;

Sdspage MW18KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details
Predicted MW: 18kd
Western blotting : 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-37) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr37) antibody (#11222, Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody (#11222).

 Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

If you have published an article using product 11222, please notify us so that we can cite your literature.

et al,Monotropein inhibits epithelial-mesenchymal transition in chronic colitis via the mTOR/P70S6K pathway. , (2025),
PMID: 40041493

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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