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Stathmin1(Phospho-Ser25) Antibody#11224

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Product Detail

Product NameStathmin1(Phospho-Ser25) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Stathmin 1 only when phosphorylated at serine 25.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 25 (I-L-S(p)-P-R) derived from Human Stathmin 1.

Target NameStathmin1

ConjugateUnconjugated

Other NamesSTMN1; STN1; stathmin

Accession NoSwiss-Prot: P16949
NCBI Protein: NP_001138926.1

Uniprot P16949

Gene ID 3925;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody #11224.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody #11224(left) or the same antibody preincubated with blocking peptide(right).
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear

Boehm M, et al. (2002) EMBO J 21(13): 3390-3401.
Vadlamudi RK, et al. (2005) Mol Cell Biol 25(9): 3726-3736.
Zilfou JT, et al. (2001) Mol Cell Biol 21(12): 3974-3985.
Biernat J, et al. (2002) Mol Biol Cell 13(11): 4013-4028.

If you have published an article using product 11224, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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