Product NameEGFR(Phospho-Tyr869) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 869 (K-E-Y(p)-H-A) derived from Human EGFR.

Target NameEGFR

ConjugateUnconjugated

Other NamesERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR

Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2

Uniprot P00533

Gene ID 1956;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 175kd
Western blotting: 1:500~1:1000