Product NameeIF4E(Phospho-Ser209) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.

Target NameeIF4E

ConjugateUnconjugated

Other NamesmRNA cap-binding protein; eIF-4F 25 kDa subunit;

Accession NoSwiss-Prot: P06730
NCBI Protein: NP_001124150.1

Uniprot P06730

Gene ID 1977;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 25kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200