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IRS-1(Phospho-Ser307) Antibody#11235

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Product Detail

Product NameIRS-1(Phospho-Ser307) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of IRS-1 only when phosphorylated at serine 307.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 307(T-E-S(p)-I-T) derived from Human IRS-1.

Target NameIRS-1

ConjugateUnconjugated

Other NamesHIRS-1

Accession NoSwiss-Prot: P35568 ; NCBI Gene ID: 3667; NCBI mRNA: NM_005544.2 ; NCBI Protein: NP_005535.1

Uniprot P35568

Gene ID 3667;

Sdspage MW180KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details
 Predicted MW: 180kd
 Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (phospho-Ser307) antibody (#11235).

 May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit 

If you have published an article using product 11235, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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