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c-Kit(Phospho-Tyr721) Antibody#11240

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Product Detail

Product Namec-Kit(Phospho-Tyr721) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 721 (N-E-Y(p)-M-D) derived from Human c-Kit.

Target Namec-Kit

ConjugateUnconjugated

Other NamesKIT , CD117, SCFR, PBT

Accession NoSwiss-Prot: P10721
NCBI Protein: NP_000213.1

Uniprot P10721

Gene ID 3815;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells untreated or treated with IFN-a using c-Kit(Phospho-Tyr721) Antibody #11240.
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
Hirota, S. et al. (1998) Science 279, 577-580.
Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

If you have published an article using product 11240, please notify us so that we can cite your literature.

et al,MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia. , (2011),
PMID: 21724256
et al,MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia. In Leuk Res on 2011 Sep by Xiao-ning Gao, Ji Lin, et al..PMID: 21724256 , (2011),
PMID: 21724256
et al,MicroRNA-193a represses c-kit expression and functions as a methylation-silenced tumor suppressor in acute myeloid leukemia. In Oncogene on 2011 Aug 4 by X-N Gao, J Lin, et al..PMID: 21399664 , (2011),
PMID: 21399664
et al,S-allyl cysteine ameliorates the quality of sperm and provides protection from age-related sperm dysfunction and oxidative stress in rats.In J Clin Biochem Nutr on 2014 Nov by Shigekazu Takemura, Hiroshi Ichikawa et al..PMID: 25411519 , (2014),
PMID: 25411519
et al,Stromal-derived MAOB promotes prostate cancergrowth and progression. In Sci Adv on 2024 Feb 9 by Tianjie Pu, Jing Wang,et al..PMID:38335292 , (2024),
PMID: 38335292

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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