Product Detail
Product NameBRCA1(Phospho-Ser1423) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 1423 (H-G-S(p)-Q-P) derived from Human BRCA1.
Target NameBRCA1
ConjugateUnconjugated
Other NamesRNF53
Accession NoSwiss-Prot: P38398
NCBI Protein: NP_009225.1
Uniprot
P38398
Gene ID
672;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using BRCA1(Phospho-Ser1423) Antibody #11242.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1(Phospho-Ser1423) Antibody #11242(left) or the same antibody preincubated with blocking peptide(right).
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation
Kim ST, et al. (2002) Genes Dev; 16(5): 560-570
Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46
Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002
If you have published an article using product 11242, please notify us so that we can cite your literature.
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